Part:BBa_K2092004

alcR (incl RBS), ethanol-activated transcription factor from A. nidulans

This part encodes for the alcR gene, which is a positive regulatory gene in the ethanol regulon of filamentous fungus Aspergillus nidulans (A.nidulans). It encodes the AlcR protein, an ethanol-activated transcription factor which would bind to its target promoters, including the alcA (PalcA, https://parts.igem.org/Part:BBa_K2092002) and aldA promoters.

AlcR is also known as a zinc binuclear cluster activator as it contains a DNA-binding domain belonging to the C6 zinc binuclear cluster family. AlcR is unique in that it can bind to symmetric and asymmetric DNA sites with the same apparent affinity and also bind to single site with high affinity [1].

A strong RBS (BBa_J34801, https://parts.igem.org/Part:BBa_J34801) has been added upstream of the start codon.

Usage and Biology

This part is useful as an ethanol reporter.

Reference

[1] Felenbok, B., Sequeval, D., Mathieu, M., Sibley, S., Gwynne, D.I. and Davies, R.W. (1988). The ethanol regulon in Aspergillus nidulans: Characterization and sequence of the positive regulatory gene alcR. Gene, 73(2), 385–396.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2568
  Illegal BamHI site found at 1572
  Illegal BamHI site found at 2310
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1276
  Illegal AgeI site found at 1404
  Illegal AgeI site found at 1935
  
• 1000
  COMPATIBLE WITH RFC[1000]